ANSWER 4<\/span><\/span><\/p>\n <\/span><\/p>\n W<\/span>et preps <\/span>are Under low power, observe for:<\/span><\/p>\n Under high power, observe for:<\/span><\/p>\n <\/p>\n K<\/span>OH<\/span> prep is made <\/span><\/p>\n W<\/span>hiff<\/span> test may be <\/p>\n p<\/span>H <\/span>test is <\/p>\n <\/span><\/p>\n <\/p>\n","protected":false},"excerpt":{"rendered":" VAGINITIS CASE 1 ANSWER 4 Wet preps are obtained using a Q-tip applied to the vaginal side-wall within the largest pool of discharge. Drop the Q-tip with the sample of discharge into a test tube with approximately 2 ml of normal saline (not water) in it. A drop of the suspension is then….<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":1565,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"page-nosidebar.php","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"class_list":["post-1575","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/jacobimed.org\/old\/wp-json\/wp\/v2\/pages\/1575","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/jacobimed.org\/old\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/jacobimed.org\/old\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/jacobimed.org\/old\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/jacobimed.org\/old\/wp-json\/wp\/v2\/comments?post=1575"}],"version-history":[{"count":1,"href":"https:\/\/jacobimed.org\/old\/wp-json\/wp\/v2\/pages\/1575\/revisions"}],"predecessor-version":[{"id":1577,"href":"https:\/\/jacobimed.org\/old\/wp-json\/wp\/v2\/pages\/1575\/revisions\/1577"}],"up":[{"embeddable":true,"href":"https:\/\/jacobimed.org\/old\/wp-json\/wp\/v2\/pages\/1565"}],"wp:attachment":[{"href":"https:\/\/jacobimed.org\/old\/wp-json\/wp\/v2\/media?parent=1575"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\nobtained using a Q-tip applied to the vaginal side-wall within the largest pool
\nof discharge. Drop the Q-tip with the sample of discharge into a test tube
\nwith approximately 2 ml of normal saline (not water) in it. A drop of the
\nsuspension is then placed on a slide, covered with a cover-slip, and carefully
\nexamined.<\/span>\n<\/p>\n\n
![\"\/public\/images\/ambulatory_content\/tvaginalis_1.gif\"](\"\/public\/images\/ambulatory_content\/tvaginalis_1.gif\" "\\"\/public\/images\/ambulatory_content\/tvaginalis_1.gif\\"")
are motile pear-shaped organisms with active flagella,
\n larger than a WBC but smaller than epithelial cells, that usually are seen
\n swimming or thrashing around in the wet prep. <\/span><\/li>\n
\n of PMNs indicates inflammation which is seen more with trichomoniasis,
\n cervicitis, and to some degree with atrophic vaginitis.<\/span><\/li>\n
\n rectangular or sharp-edged, but an atrophic vagina sheds round or oval
\n cells. <\/span><\/li>\n<\/ul>\n\n
![\"\/public\/images\/ambulatory_content\/clue.4.jpg\"](\"\/public\/images\/ambulatory_content\/clue.4.jpg\" "\\"\/public\/images\/ambulatory_content\/clue.4.jpg\\"")
these are epithelial cells that have tiny pleomorphic bacteria adherent to
\n their surfaces, obscuring their borders and causing a stippled appearance. <\/span><\/li>\n![\"\/public\/images\/ambulatory_content\/hyphae.jpg\"](\"\/public\/images\/ambulatory_content\/hyphae.jpg\" "\\"\/public\/images\/ambulatory_content\/hyphae.jpg\\"")
<\/span><\/li>\n
\n flora. ![\"\/public\/images\/ambulatory_content\/lactobacillus.1.gif\"](\"\/public\/images\/ambulatory_content\/lactobacillus.1.gif\" "\\"\/public\/images\/ambulatory_content\/lactobacillus.1.gif\\"")
<\/span><\/li>\n<\/ul>\n
\nby adding a drop of 10% KOH solution to a drop of saline suspension of the
\ndischarge. The KOH lyses epithelial cells in 5 minutes (faster if the slide is
\nwarmed briefly over a flame) and allows easier microscopic visualization of
\nCandidal hyphae. Other fungal stains may also be used. Swartz-Lamkin
\nstain contains a base for lysis of epithelial cells and india ink, which is
\ntaken up by fungal cells. <\/span><\/p>\n
\ndone either by adding a few drops of KOH to the vaginal discharge remaining on
\nthe lip of the speculum or by sniffing the KOH slide just after the KOH is
\nadded. A foul, fishy odor is indicative of anaerobic overgrowth and, thus,
\nbacterial vaginosis (though trichomonas can cause false positivies).<\/span><\/p>\n
\nperformed by dipping pH paper into the discharge remaining on the vaginal
\nspeculum (before doing the whiff test)<\/span><\/p>\n